個人資訊 ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

學    歷  ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

國立清華大學 生命科學系 博士

台北醫學大學 基礎醫學研究所 碩士

台北醫學大學 藥學系 學士

經    歷 ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

國立清華大學 分子與細胞生物研究所

國立清華大學 生物資訊與結構生物研究所

林口長庚大學 血液腫瘤科

研究領域 ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

生物資訊、蛋白質結構、藥物設計、液體半固體製劑劑型

(1)利用蛋白質結構探討酵素與其活性間的關係

    蛋白質為生物體內用來進行細胞代謝，當蛋白質受影響時其功能就會變差。這些重要蛋白質分子的催化機制與其結構有相當大的關係，蛋白質的活性區可提供有利催化受質的環境以利反應的進行，活性區內的重要的胺基酸會與受質結合產生化學反應並生成產物，所以利用蛋白質結構來探討其與活性間的關係，進一步利用蛋白質工程的方法改造蛋白質，演化出新酵素並應用在醫學、環保、農業和藥物的改良上。

(2)利用生物資訊方法進行藥物虛擬篩選

    生物資訊學為應用數學、資訊學、統計學和計算機科學的方法來研究生物，其方法包括對生物學數據的尋找、整理及利用。我的研究重點在生物基因的序列分析(基因組注釋)、比較基因組學和後端藥物標靶藥物虛擬篩選，利用三度空間蛋白質藥物複合體晶體結構的解析分析其藥效基團藥物進行循理性藥物設計研發。

期刊論文  ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

1. Chun-Ying Huang, Yi-Hsiu Tsai, Yi-Fen Cheng, Peng-Yu Wu, Yu-Chi Chuang, Po-Yuan Huang, Jai-Shin Liu, Chen-Chi Wu & Yen-Fu Cheng. CRISPR/Cas9-mediated exon skipping to restore premature translation termination in a DFNB4 mouse model. Gene Ther 04 September 2024.

2. Jai‑Shin Liu, Wei‑Kai Fang, Shan‑Min Yang, Meng‑Chen Wu, Tsan‑Jan Chen, Chih‑Ming Chen, Tung‑Yueh Lin, Kai‑Lun Liu, Chien‑Ming Wu, Yun‑Ching Chen, Chih‑Pin Chuu, Ling‑Yu Wang. Hsing‑Pang Hsieh3,4,9, Hsing‑Jien Kung10,11 and Wen‑Ching Wang1Natural product myricetin is a pan‑KDM4 inhibitor which with poly lactic‑co‑glycolic acid formulation effectively targets castration‑resistant prostate cancer Journal of Biomedical Science (2022) 29:29(IF:11(2022))

3. Sankhya Bhattacharya, Pijus Kundu, J.S. Liu, Wen-Ching Wang, and Fan-Gang Tseng. Feedback-System-Control Integrated Microfluidic System for Fast Screening of Protein Crystallization Conditions. Cryst. Growth Des. 2020, 20, 4325−4334.

4. Chen TJ, Wang HJ, Liu JS, Cheng HH, Hsu SC, Wu MC, Lu CH, Wu YF, Wu JW, Liu YY, Kung HJ, Wang WC. Mutations in the PKM2 exon-10 region are associated with reduced allostery and increased nuclear translocation. Commun Biol. 2019 Mar 15;2:105. doi: 10.1038/s42003-019-0343-4. eCollection 2019.

5. Ying-Yu Kuo, Chieh Huo, Ching-Yu Lin, Hui-Ping Lin, Jai-Shin Liu, Wen-Ching Wang,Chuang-Rung Chang and Chih-Pin Chuu. Caffeic acid phenethyl ester suppresses androgen receptor signaling and stability via inhibition of phosphorylation on Ser81and Ser213. Cell Communication and Signaling (2019) 17:100. https://doi.org/10.1186/s12964-019-0404-9

6. Lai CH, Huang JC, Cheng HH, Wu MC, Huang MZ, Hsu HY, Chen YA, Hsu CY, Pan YJ, Chu YT, Chen TJ, Wu YF, Sit WY, Liu JS, Chiu YF, Wang HJ, Wang WC. Helicobacter pylori cholesterol glucosylation modulates autophagy for increasing intracellular survival in macrophages. Cell Microbiol. 2018 Dec;20(12):e12947. doi: 10.1111/cmi.12947. Epub 2018 Sep 17.

7. Jai-Shin Liu. Yi-Chia Kuan, Yu Tsou, Tung-Yueh Lin, Wen-Hwei Hsu, Ming-Te Yang, Jong-Yih Lin, and Wen-Ching Wang. Structure-guided design of Serratia marcescens short-chain dehydrogenase/reductase for stereoselective synthesis of (R)-phenylephrine. Sci. Rep. (2018) 8:2316 | DOI:10.1038/s41598-018-19235-y

8. Yi-Chia Kuan, Venkatesan Thiruvengadam, Jia-Shin Lin, Jia-Hsin Liu, Tsan-Jan Chen, Hsin-Mao Wu, Wen-Ching Wang, Liang-Jwu Chen. Broad-specificity amino acid racemase, a novel non-antibiotic selectable marker for transgenic plants, Plant Biotechnology Reports, pp 1–12,

9. Cheng-Lung Hsu, Jai-Shin Liu, Ting-Wei Lin, Ying-Hsu Chang, Yung-Chia Kuo, An-Chi Lin, Huei-Ju Ting, See-Tong Pang, Li-Yu Lee, Wen-Lung Ma, Chun- Cheng Lin and Wen-Guey Wu. Characterization of a novel androgen receptor (AR) coregulator RIPK1 and related chemicals that suppress AR-mediated prostate cancer growth via peptide and chemical screening. Oncotarget. 2017: 8(41), 69508-69519

10. Sheng-Chia Chen, Chi-Hung Huang, Shu-Jung Lai, Jai-Shin Liu, Pin-Kuei Fu,Shih-Ting Tseng, Chia Shin Yang, Mei-Chin Lai, Tzu-Ping Ko & Yeh Chen. Structure and mechanism of an antibiotics-synthesizing 3-hydroxykynurenine C-methyltransferase, Scientific Report, 2015,May 11.

11. Cheng-Lung Hsu, Jai-Shin Liu, Po-Long Wu, Hong-Hsiang Guan, Yuh-Ling Chen, An-Chi Lin, Huei-Ju Ting, See-Tong Pang, Shauh-Der Yeh, Wen-Lung Ma, Chung-Jung Chen, Wen-Guey Wu, Chawnshang Chang. Identification of a new androgen receptor (AR) co-regulator BUD31 and related peptides to suppress wild-type and mutated AR-mediated prostate cancer growth via peptide screening and X-ray structure analysis December 2014, Pages 1575–1587, Molecular Oncology.

12. Cheng-Lung Hsu, Jai-Shin Liu, An-Chi Lin, Chih-Hsun Yang, Wen-Hung Chung, Wen-Guey Wu Minoxidil may suppress androgen receptor-related functions, Oncotarget, Vol. 5, No. 8, 2187-97. April 08, 2014.

13. Sheng-Chia Chen, Chi-Hung Huang, Chia Shin Yang, Jai-Shin Liu, Shu-Min Kuan and Yeh Chen. Crystal structures of the archaeal UDP-GlcNAc 2-epimerase from Methanocaldococcus jannaschii reveal a conformational change induced by UDP-GlcNAc. Proteins: Structure, Function and Bioinformatics.

14. Je-Hung Kuo, Ya-Ping Chen, Jai-Shin Liu, Alexandre Dubrac, Cathy Quemener, Hervé Prats4Andreas Bikfalvi, Wen-guey Wu, and Shih-Che Sue. Alternative C-Terminal Helix Orientation Alters Chemokine Function: Structure of the Antiangiogenic Chemokine, CXCL4L1. J Biol Chem. 2013 May 10; 88 19):13522-33. 2013 Mar 27.

15. Yu-Ling Wang, Je-Hung Kuo, Shao-Chen Lee, Jai-Shin Liu, Yin-Cheng Hsieh, Yu-Tsung Shih, Chun-Jung Chen, Jeng-Jiann Chiu, and Wen-guey Wu. Cobra CRISP functions as an inflammatory modulator via a novel Zn2+- and heparan sulfate- dependent transcriptional regulation of endothelial cell adhesion molecules. J. Biol. Chem. 285,No, 48,pp 37872–37883. 2010

16. Shu-Chen Kan, Jai-Shin Liu , Hui-Yu Hu , Chia-Ming Chang , Wei-De Lin, Wen-Ching Wang , Wen-Hwei Hsu Biochemical characterization of two thymidylate synthases in Corynebacterium glutamicum NCHU 87078. Biochimica et Biophysica Acta 1804 (2010) 1751–1759.

17. Liu JS, Cheng WC, Wang HJ, Chen YC, Wang WC (2008). Structure-based inhibitor discovery of Helicobacter pylori dehydroquinate synthase. Biochem. Biophys. Res.Commun. 373 (2008) 1–7.

18. Long-Liu Lin, Jai-Shin Liu, Wen-Ching Wang, Szu-Han Chen, Chih-Chen Huang, Huei-Fen Lo. Glutamic acid 219 is critical for the thermostability of a truncated a-amylase from alkaliphilic and thermophilic Bacillus sp. Strain TS-23. World J Microbiol Biotechnol (2008) 24:619–626.

19. Hung CL, Liu JH, Chiu WC, Huang SW, Hwang JK, and Wang WC. Crystal structure of Helicobacter pylori formamidase AmiF reveals a cysteine-glutamate-lysine catalytic triad. (2007). J. Biol. Chem. 282, 12220–12229.

20. Chi MC, Liu JS, Wang WC, Lin LL*, Hsien-Ben Hwang (2007). Site-directed mutagenesis of the conserved Ala348 and Gly350 residues at the putative active site of Bacillus kaustophilus leucine aminopeptidase. Biochimie. 90(2008) 811-819.

21. Chiu WC, You JY, Liu JS, Hsu SK, Hsu WH, Shih CH, Hwang JK, Wang WC Structure-stability-activity relationship in covalently cross-linked N-carbamoyl D-amino acidamidohydrolase and N-acylamino acid racemase (2006). J. Mol. Biol. 359, 741–753.

22. Lin LL, Chen PI, Liu JS, Wang WC, Lo HF (2006). Identification of glutamate residuesimportant for catalytic activity or thermostability of a truncated Bacillus sp. strain TS-23α-amylase by site-directed mutagenesis. Protein J. 25, 232–239.

23. Chi MC, Huang HB, Liu JS, Wang WC, Liang WC & Lin LL. Residues threonine 346 and leucine 352 are critical for the proper function of Bacillus kaustophilus leucine aminopeptidase (2006). FEMS Microbiol. Lett. 260, 156–161.

24. Wang WC, Chiu WC, Hsu SK, Wu CL, Chen CY, Liu JS, and Hsu WH (2004). Structural basis for catalytic racemization and substrate specificity of an N-acylamino acid racemase homologue from Deinococcus radiodurans. J. Mol. Biol. 342, 155–169.

25. Chen CY, Chiu WC, Liu JS, Hsu WH, Wang WC (2003). Structural basis for catalysis and substrate specificity of Agrobacterium radiobacter N-carbamoyl-D-amino-acidamidohydrolase. J. Biol. Chem. 278, 26194–26201.