個人資訊  ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

實驗室網址

學    歷  ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

國立清華大學 生命科學系 博士 (2003)

國立清華大學 生命科學系 碩士 (1990)

中原大學 物理系 學士 (1988)

經    歷  ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

現職 元培生物科技系 副教授

         光宇檢驗中心主任(03-6102450)

元培科技大學技術研發管理中心中心 主任

元培科技大學貴重儀器中心 主任

工業技術研究院生醫工程中心顧問(幹細胞與細胞外間質研究)

國立清華大學生物資訊與結構生物研究所 博士後研究

華生藥物科技公司 研發部副理

圓二色光譜估算蛋白質二級結構程式建立 (國家同步輻射研究中心 產學合作) CD_Fit5

研究領域  ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

Biophysics / Membrane / Bio-spectroscopy / Structural Biology

(A) 蛋白質、天然毒素結構與功能分析

大自然中許多生物具有毒性其目的就是利用微量的毒素攻擊其他生物的生命系統，但所謂的藥/毒一家，最具致命毒性的物質也是最有可能成為救命的良藥。例如治療血管血栓的藥物就是來自會讓人血流不止的蛇毒素，現在最流行的美容針肉毒桿菌素則是利用其神經麻痺功能來達到除皺紋，蜜蜂尾針造成強烈痛楚的蜂毒素則有人嘗試做為人體免疫功能的調節分子，因此了解各類生物毒素的結構與功能並進一步發展可能新藥物便是目前的研究方向。

(B) 生物分子光譜研究

生物分子例如蛋白質、核酸、細胞膜與醣類皆是奈米等級的大小，無法以一般的光學顯微鏡或是儀器觀察到，因此透過x-光晶體繞射、紫外線圓二色光譜、紅外光、電子共振自旋光譜或是核磁共振將可以量測到分子的結構或是動態行為，也方更了解生物的運作。微生物製造長鏈二元酸只需要在室溫及一般大氣壓下便可進行，但是生物體中有各式各樣的酵素同步進行著各式反應，這些反應不一定會依照我們的意願完美的進行，因此分析烴烷原料在微生物中轉換為二元酸及其副產物的比例與數量便是了解與未來進一步調控微生物發酵過程的必要工具。

實驗室技術

手機檢測農藥殘留(Lab在我家)，生物紅外光譜，螢光光譜及單層分子薄膜，圓二色光譜(Circular Dichroism) 磷脂水解酶A2，電腦模擬，原子力顯微鏡。

計畫與合作案

期刊論文 ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

1.     Wu PL, Chiu CR, Huang WN*, Wu WG*. The role of sulfatide lipid domains in the membrane pore-forming activity of cobra cardiotoxin. Biochim Biophys Acta., 1818:1378-85, 2012(SCI)

2.     Huang WN*, Chen YH, Chen CL, Wu W* Surface pressure-dependent interactions of secretory phospholipase A2 with zwitterionic phospholipid membranes Langmuir, 27:7034-41, 2011.(SCI)

3.     Lin ZS, Lo FC, Li CH, Chen CH, Huang WN, Hsu IJ, Lee JF, Horng JC, Liaw WF* Peptide-Bound Dinitrosyl Iron Complexes (DNICs) and Neutral/Reduced-form Roussin’s Red Esters (RREs/rRREs) : Understanding Nitrosylation of [Fe-S] Clusters Leading to the Formation of DNICs and RREs Using a De Novo Design Strategy.Inorganic Chemistry, 50:10417-31, 2011.(SCI)

4.     Chang HH, Huang HJ, Ho YL, Wen YD, Huang WN, Chiou SJ.* The water-soluble Roussin's red ester acting as a potential photochemical NO-delivery agent: photolysis reactions, DNA cleavage and anticancer activity. Dalton Trans., 28, P. 6396-6402,2009.(SCI)

5.     Chang-Ru Chiu, Wei-Ning Huang, Wen-Guey Wu,* and Tzyy-Schiuan Yang*Fluorescence Single-Molecule Study of Cobra Phospholipase A2 Action on a Supported Gel-Phase Lipid Bilayers Chemphyschem, 10, P.549-558, 2009.(SCI)

6.     Tjong SC, Chen TS, Huang WN, Wu WG. (2007) Structures of heparin-derived tetrasaccharide bound to cobra cardiotoxins: heparin binding at a single protein site with diverse side chain interactions. Biochemistry.;46(35):9941-52.

7.     Ho MR, Lou YC, Lin WC, Lyu PC, Huang WN, Chen C. (2006) Human pancreatitis-associated protein forms fibrillar aggregates with a native-like conformation. J Biol Chem.;281(44):33566-76.

8.     Chen TS, Chung FY, Tjong SC, Goh KS, Huang WN, Chien KY, Wu PL, Lin HC, Chen CJ, Wu WG. (2005) Structural difference between group I and group II cobra cardiotoxins: X-ray, NMR, and CD analysis of the effect of cis-proline conformation on three-fingered toxins. Biochemistry. 44,7414-7426.

9.     Lee SC, Guan HH, Wang CH, Huang WN, Tjong SC, Chen CJ, Wu WG. (2005) Structural basis of citrate-dependent and heparan sulfate-mediated cell surface retention of cobra cardiotoxin A3. J Biol Chem. 280, 9567-77.

10.   Chen TS, Chung FY, Tjong SC, Goh KS, Huang WN, Chien KY, Wu PL, Lin HC, Chen CJ, Wu WG. (2005) Structural difference between group I and group II cobra cardiotoxins: X-ray, NMR, and CD analysis of the effect of cis-proline conformation on three-fingered toxins. Biochemistry. 44, 7414-26.

11.   Huang WN, Sue SC, Wang DS, Wu PL, Wu WG. (2003) Peripheral Binding Mode and Penetration Depth of Cobra Cardiotoxin on Phospholipid Membranes as Studied by a Combined FTIR and Computer Simulation Approach. Biochemistry.42, 7457-7466.

12.   Forouhar F, Huang WN, Liu JH, Chien KY, Wu WG, Hsiao CD. (2003) Structural Basis of Membrane-induced Cardiotoxin A3 Oligomerization. J Biol Chem. 278, 21980-21988.

13.   Wu CW, Cheng SF, Huang WN, Trivedi VD, Veeramuthu B, Assen B K, Wu WG, Chang DK. (2003) Effects of alterations of the amino-terminal glycine of influenza hemagglutinin fusion peptide on its structure, organization and membrane interactions. Biochim Biophys Acta. 1612, 41-51.

14.   Sue SC, Chien KY, Huang WN, Abraham JK, Chen KM, Wu WG. (2002) Heparin binding stabilizes the membrane-bound form of cobra cardiotoxin. J Biol Chem. 277, 2666-2673.

15.   Sue SC, Brisson JR, Chang SC, Huang WN, Lee SC, Jarrell HC, Wu W. (2001) Structures of heparin-derived disaccharide bound to cobra cardiotoxins: context-dependent conformational change of heparin upon binding to the rigid core of the three-fingered toxin. Biochemistry. 40, 10436-10446.

16.   Lin YH, Huang WN, Lee SC, Wu WG. (2000) Heparin reduces the alpha-helical content of cobra basic phospholipase A2 and promotes its complex formation. Int J Biol Macromol. 27, 171-176.

17.   Guo FC, Chou YC, Huang WN, Wu WG. (1992) SERS (surface enhanced Raman scattering) study of the conformational transition of the ordering of lipid molecules deposited on a sliver electrode. J. Raman Spectr.23, 425-430.

18.   Chien KY, Huang WN, Jean JH, Wu WG. 1991. Fusion of sphingomyelin vesicles induced by proteins from Taiwan cobra (Naja naja atra) venom. Interactions of zwitterionic phospholipids with cardiotoxin analogues. J Biol Chem. 266, 3252-3259.