個人資訊  ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

實驗室網址

學    歷  ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

國立清華大學 生命科學系 博士 (2003)

國立清華大學 生命科學系 碩士 (1990)

中原大學 物理系 學士 (1988)

經    歷  ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

 研究領域   ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

Biophysics / Membrane / Bio-spectroscopy / Structural Biology

(A) 蛋白質、天然毒素結構與功能分析

大自然中許多生物具有毒性其目的就是利用微量的毒素攻擊其他生物的生命系統，但所謂的藥/毒一家，最具致命毒性的物質也是最有可能成為救命的良藥。例如治療血管血栓的藥物就是來自會讓人血流不止的蛇毒素，現在最流行的美容針肉毒桿菌素則是利用其神經麻痺功能來達到除皺紋，蜜蜂尾針造成強烈痛楚的蜂毒素則有人嘗試做為人體免疫功能的調節分子，因此了解各類生物毒素的結構與功能並進一步發展可能新藥物便是目前的研究方向。

(B) 生物分子光譜研究

生物分子例如蛋白質、核酸、細胞膜與醣類皆是奈米等級的大小，無法以一般的光學顯微鏡或是儀器觀察到，因此透過x-光晶體繞射、紫外線圓二色光譜、紅外光、電子共振自旋光譜或是核磁共振將可以量測到分子的結構或是動態行為，也方更了解生物的運作。微生物製造長鏈二元酸只需要在室溫及一般大氣壓下便可進行，但是生物體中有各式各樣的酵素同步進行著各式反應，這些反應不一定會依照我們的意願完美的進行，因此分析烴烷原料在微生物中轉換為二元酸及其副產物的比例與數量便是了解與未來進一步調控微生物發酵過程的必要工具。

實驗室技術

手機檢測農藥殘留(Lab在我家)，生物紅外光譜，螢光光譜及單層分子薄膜，圓二色光譜(Circular Dichroism) 磷脂水解酶A2，電腦模擬，原子力顯微鏡。

計畫與合作案  ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

 期刊論文  ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

1. Yi-Huang Chang,Wenni Huang,Hong-Jun Chen,Chih-Cheng Lin，The composition and health benefits of mackerel concentrate. MOJ Food Processing & Technology 2019 Jul 7(3) 88-90

2. 黃泰郎;黃維寧*，眼球結膜紫外線螢光影像之初期研究 The Preliminary Study of Ultraviolet Fluorescence Photography on Eye Conjunctiva. TOVS, 2016; 1(2):45-49

3. Lo FC, Hsieh CC, Maestre-Reyna M, Chen CY, Ko TP, Horng YC, Lai YC, Chiang YW, Chou CM, Chiang CH, Huang WN, Lin YH, Bohle DS, Liaw WF. Crystal Structure Analysis of the Repair of Iron Centers Protein YtfE and Its Interaction with NO. Chemistry. 2016 Jul 4;22(28):9768-76. (SCI)

4. 吳柏龍 ; 陳錦坤 ; 涂茂園 ; 黃維寧 ; 莊路德 長鏈二元酸分子定性與定量方法的建立與評估=Establishment of Qualitative and Quantitative Methods for Long-chain Dicarboxylic Acids 石油季刊 50:4 2014.12[民103.12] 頁93-105

5. Huang WN, Yang CY, Chen DC, Chuang LT CORRELATION OF THE STRUCTURE AND BIOACTIVITY OF RECOMBINANT FUNGAL IMMUNOMODULATORY PROTEIN, LING-ZHI-8 (LZ-8) FOLLOWING EXPOSURE TO DENATURING CONDITIONS. J. Food Biochem. 2014 38,328–36(SCI)

6. Chen JC, Chiu LL, Lee KL, Huang WN, Chuang JG, Liao HK, Chow LP. Identification of critical amino acids in an immunodominant IgE epitope of Pen c 13, a major allergen from Penicillium citrinum. PLoS One. 2012;7(4):e34627. (SCI)

7. Wu PL, Chiu CR, Huang WN*, Wu WG*. The role of sulfatide lipid domains in the membrane pore-forming activity of cobra cardiotoxin. Biochim Biophys Acta., 1818:1378-85, 2012(SCI)

8. Huang WN*, Chen YH, Chen CL, Wu W* Surface pressure-dependent interactions of secretory phospholipase A2 with zwitterionic phospholipid membranes Langmuir, 27:7034-41, 2011.(SCI)

9. Lin ZS, Lo FC, Li CH, Chen CH, Huang WN, Hsu IJ, Lee JF, Horng JC, Liaw WF* Peptide-Bound Dinitrosyl Iron Complexes (DNICs) and Neutral/Reduced-form Roussin’s Red Esters (RREs/rRREs) : Understanding Nitrosylation of [Fe-S] Clusters Leading to the Formation of DNICs and RREs Using a De Novo Design Strategy.Inorganic Chemistry, 50:10417-31, 2011.(SCI)

10. Chang HH, Huang HJ, Ho YL, Wen YD, Huang WN, Chiou SJ.* The water-soluble Roussin's red ester acting as a potential photochemical NO-delivery agent: photolysis reactions, DNA cleavage and anticancer activity. Dalton Trans., 28, P. 6396-6402,2009.(SCI)

11. Chang-Ru Chiu, Wei-Ning Huang, Wen-Guey Wu,* and Tzyy-Schiuan Yang*Fluorescence Single-Molecule Study of Cobra Phospholipase A2 Action on a Supported Gel-Phase Lipid Bilayers Chemphyschem, 10, P.549-558, 2009.(SCI)

12. Tjong SC, Wu PL, Wang CM, Huang WN, Ho NL, Wu WG.* Role of glycosphingolipid conformational change in membrane pore forming activity of cobra cardiotoxin. Biochemistry., 46 P.12111-12123, 2007.(SCI)

13. Tjong SC, Chen TS, Huang WN, Wu WG* Structures of heparin-derived tetrasaccharide bound to cobra cardiotoxins: heparin binding at a single protein site with diverse side chain interactions. Sep 4;Biochemistry., 46 ,9941-9952., 2007.(SCI)

14. Ho MR、Ho MR、Lou YC、Lin WC、Lyu PC、Huang WN、Chen C 2006,11 Human pancreatitis-associated protein forms fibrillar aggregates with a native-like conformation J Biol Chem (SCI) 281卷第44期 33566-76

15. Chen TS, Chung FY, Tjong SC, Goh KS, Chien KY, Huang, WN, Wu PL, Lin HC, Chen CJ, Wu WG. 2005,5 Structural difference between group I and group II cobra cardiotoxins: X-ray, NMR, and CD analysis of the effect of cis-proline conformation on three-fingered toxins. Biochemistry (SCI) 44(20) 7414-7426

16. Lee SC, Guan HH, Wang CH, Huang WN, Tjong SC, Chen CJ, Wu WG. 2005,3 Structural basis of citrate-dependent and heparan sulfate-mediated cell surface retention of cobra cardiotoxin A3. Journal of Biological Chemistry (SCI) 280(10) 9567-9577

17. Forouhar Farhad,Huang, Wei-Ning, Liu Jyung-Hurng, Chien Kun-Yi, Wu Wenguey, Hsiao Chwan-Deng. 2003,6 Structural Basis of Membrane-induced Cardiotoxin A3 Oligomerization, Journal of Biological Chemistry (SCI) Vol.278 pp21980-21988

18. Huang, Wei-Ning, Sue Shih-Che, Wang Da-Shin, Wu Po-Long, Wu Wenguey. 2003,6 Peripheral Binding Mode and Penetration Depth of Cobra Cardiotoxin on Phospholipid Membranes as Studied by a Combined FTIR and Computer Simulation Approach, Biochemistry (SCI) Vol.42 pp.7457-7466

19. Wu Cheng-Wei, Cheng Shu-Fang, Huang, Wei-Ning, Trivedi Vishwa Deo, Veeramuthu Balakrishnan, Assen B Kantchev, Wu Wenguey, Chang Ding-Kwo. 2003,5 Effects of Alterations of the Amino-Terminal Glycine of Influenza Hemagglutinin Fusion Peptide on its Structure, Organization and Membrane Interactions, Biochimica Biophysica Acta.-Biomembranes (SCI) Vol.1612 pp41-51

20. Sue Shih-Che, Chien Kun-Yi, Huang, Wei-Ning, Abraham Joseph K, Chen Kuan-Ming, Wu Wenguey. 2002,1 Heparin binding stabilizes the membrane-bound form of cobra cardiotoxin, Journal of Biological Chemistry (SCI) Vol.277 pp2666-2673

21. Sue Shih-Che, Brisson Jean-R, Chang Sho-Chan, Huang, Wei-Ning, Lee Shao-Chen, Jarrell HC, Wu Wenguey. 2001,9 Structures of Heparin-Derived Disaccharide Bound to Cobra Cardiotoxins: Context-Dependent Conformational Change of Heparin upon Binding to the Rigid Core of the Three-Fingered Toxin, Biochemistry (SCI) Vol.40 pp10436-10446

22. Lin Yi-Hung, Huang, Wei-Ning, Lee Shao-Chen, Wu Wenguey. 2000,4 Heparin Reduces the Alpha-Helical Content of Cobra Basic Phospholipase A2 and Promotes its Complex Formation, International Journal of Biological Macromolecules (SCI) Vol.27 pp171-176.

23. Guo Fan-Chei, Chou Yar-Chen, Huang, Wei-Ning, Wu Wenguey. 1992,3 SERS (Surface Enhanced Raman Scattering) Study of the Conformational Transition of the Ordering of Lipid Molecules Deposited on a Sliver Electrode,Journal of Raman Spectroscopy (SCI) Vol.23 pp425-430

24. Chien Kun-Yi, Huang, Wei-Ning, Jean Jau-Hua, Wu Wenguey. 1991, 2 Fusion of Sphingomyelin Vesicles Induced by Proteins from Taiwan Cobra (Naja naja atra) Venom. Interactions of Zwitterionic Phospholipids with Cardiotoxin Analogues, Journal of Biological Chemistry (SCI) Vol.266 pp3252-3259

學術研討會 ╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴╴

1. 林芷萱、黃維寧 2019,08,21 用智慧型手機裝置進行農藥快篩2019食品衛生檢驗科技研討會 PC-15 嘉義食安大樓, 台灣

2. H.W.Fu1, Y.H. Lin, L.J.Huang, H.S.Fung, S.C.Chun, C.T.Chen and W-N Huang 2015, 5,17 Dynamic synchrotron radiation circular dichroism beamline BL04C1 at Taiwan light source. 590.WE-Heraeus-Seminar on Synchrotron Radiation Circular Dichroism Spectroscopy. Physikzentrum Bad Honnef, Germany (oral)

3. Yi-Hsun Tsao、Je-Hung Guo、Wei-Ning Huang、Wen-guey Wu 2015 2,7 PI(4,5)P2 lipid binding induced a reorientation of FGF2 molecules near membrane surface to facilitate the unconventional oligomerization-dependent secretion process as revealed by a combined FTIR/NMR/X-ray study. Biophysical Society 59th Annual Meeting Baltimore, Maryland, US

4. 林惠敏、黃維寧 2014,10,25 以統計方法量測及評估落髮與頭髮生長之狀態 健康管理學術研討會

5. Wei-Ning Huang、Chun Guan、Yi-Hun Lin 2013,6,27 CD_Fit5: A Combination of Match and Linear Regression Methods to estimate Protein Secondary Structure from Circular Dichroism Spectr. The 18th Biophysics Conference

6. Tzu-Yu Wang、Chou-Chun Huang、Yi-Hsun Tsao、Tswen-Kei Tang、Wei-Ning Huang、Yi-Hung Lin、Yung-Hsiao Chen、Shih-Fang Huang 2011,12,15 Revealing the Catalytic site and Metal Ion Selectivity of the Monomeric Glyoxalase I from Oryza sativa 2011 Taiwan Enzyme Mechanism Conference. 2011年台灣酵素機制研討會 1 178

7. 江智瑜、楊均威、楊譽禎、鄒國鳳、黃維寧、柯淑婷、周瑞玲 2011,10,29 溫度對感溫性水膠之蛋白質釋放行為的影響 2011健康與管理學術研討會 YPU, Hsinchu, Taiwan 20

8. Yi-Hsum Tsao、We-Ning Huang、Chia-Hsin Liu 2011,10,19 The study of phospholipase A2 and inhibitors interaction Seventeenth User's meeting & Workshop NSRRC, Hsinchu, Taiwan 197

9. Wei-Ning Huang、Yi-Hung Lin、Yi-Hsun Tsao、Chun-Yu Kao、Chen Kao、Meng-Han Liu、Chen-Hua Huang 2010,10,20 Characterization of Protein Secondary Structure at Dry Film and Solution by SRCD and ATR-FTIR Sisteenth Users' Meeting (NSRRC) NSRRC, Hsinchu, Taiwan 183

10. Wei-Ning Huang、Wu Wen-guey 2009,11,16 Single molecule and X-ray crystallographic studies on the interfacial action mechanism of phospholipase A2 on phospholipid membranes Institute for Protein Research retreat 2009 Awaji, Osaka, Japan 38 (oral)

11. Wei-Ning Huang、Yi-Hsun Tsao、Yung-Hsiao Chen、Y-Hung Lin 2009,10,15 The Structure and Functional Studies of BAX Protein in Artificial Membranes Fifteenth Users' Meeting (National Synchrotron ZRadiation Research Center) NSRRC, Hsinchu, Taiwan 177

12. Weu-Ning Huang、Yi-Huang Yeh、C-R Chiu、T-S Yang、C-D Haiao、W-G Wu 2008,12,16 Substrate binding intermediates for interfacial enzyme activation of cobra phospholipase A2 by phosphatidylcholine micelles Institute for Protein Research Retreat 200 道場 生野字, Osaka, Japan 86

13. Wei-Ning Huang、Chia-Lu Chen 2008,10,8 Change of phospholipid bilayer induced by the hydrolytic products of hospholipase A2 The 14th User's Meeting & workshop NSRRC, HsinChu, Taiwan 146

14. Chia-Lu Chen、Huang, W-N 2008,5,21 Chang of Phospholiid Bilayer Induced by the Hydrolytic Productors of Phospholipase A2 The 13th